Lambda Legal, in collaboration with the Legal Defense Fund and other civil rights organizations, filed a motion for a preliminary injunction in National Urban League v. Trump. The action aims to halt three executive orders by President Trump that ban diversity, equity, inclusion, and accessibility and erase transgender people from public life.

On February 20, 2025, Lambda Legal represented a coalition of LGBTQ, Health, and HIV organizations in filing a lawsuit challenging three Trump-era executive orders that aim to erase transgender identities and defund essential services. This marks their continued commitment to using litigation to uphold civil rights and fight authoritarian policies.

Lambda Legal, in partnership with the Legal Defense Fund, filed a federal lawsuit challenging three executive orders from President Trump that aim to curtail diversity, equity, inclusion, accessibility programs and deny recognition to transgender people. The lawsuit argues these orders violate constitutional rights and imperil access to critical social and health services for marginalized communities.

On January 20, 2025, Lambda Legal CEO Kevin Jennings publicly condemned President Trump's executive orders targeting LGBTQ+ and other marginalized communities, vowing legal action and launching the 'Fearless + Fighting Back' campaign to challenge these measures.

Amid mounting internal turmoil marked by deep cuts to employee benefits, high staff turnover, reports of discriminatory practices (including misgendering and inadequate health coverage for transition-related care), and infighting over organizational priorities, Lambda Legal's longtime CEO Rachel Tiven resigned on August 3. The crisis underscores a troubling disconnect between the organization’s public advocacy for LGBTQ rights and its internal labor practices affecting marginalized staff.