In response to a British nursery school expelling a toddler over alleged transphobia, Sex Matters' director of advocacy, Helen Joyce, publicly criticized the educators for imposing adult ideological concepts on young children, describing the practice as 'totalitarian insanity.'

A YouGov poll commissioned by Sex Matters reveals that a clear majority of the UK public endorses amending the Equality Act to mean biological sex, reinforcing the stance that no one born male should have access to women’s spaces. This development is part of the organization's broader campaign for sex-based rights.

A YouGov poll commissioned by Sex Matters, conducted on December 19, 2024, reveals significant public support for defining sex as strictly biological in the Equality Act. The results indicate that a majority of respondents believe that individuals born male should be excluded from women’s spaces and competitive sports, reinforcing an exclusionary stance that may marginalize transgender individuals.

Sex Matters issued a formal response to the EHRC’s 2025–2028 strategic plan, presenting 10 recommendations aimed at clarifying legal definitions of sex and promoting single-sex services. Their response, which criticizes the use of the Equality Act to support gender ideology and calls for an inquiry into gender-critical belief discrimination, also aligns them with groups such as Fair Play For Women, Woman’s Place UK, Transgender Trend, and LGB Alliance. This association and their framing of policy on sex-based rights raise concerns from a progressive, anti-authoritarian perspective regarding the marginalization of transgender individuals.

Sex Matters launched a campaign letter calling on the Prime Minister to 'defend us' against the perceived politicisation of policing, criticizing the influence of gender ideology and Stonewall policies in law enforcement. The executive director, Maya Forstater, urged a review into how political involvement is impacting police impartiality and public service.